On Friday, January 29th @ 11:00AM ET, we welcome Prof. Lucio Frydman from the National High Magnetic Field Laboratory as he presents, “Water and molecular imaging: Friends or Foes?”
ABOUT THE WEBINAR
Water is the ultimate biomolecule and NMR’s most amenable target, leading to valuable information about development and physiology in health and disease (Figure 1). Molecular imaging tries to expand this scope by focusing on the metabolites and/or the macromolecules (proteins, nucleic acids, membranes) that crowd the in vivo milieu. Observing these markers via 1H-detected MR experiments is not trivial, as the dominant water signal often overwhelms the much smaller signals being sought. 1H NMR of biomolecules therefore often sees water as a foe, deserving of total, flawless suppression. The present talk will describe some experiments whereby water is actually used as a “friend”, to facilitate the observation of structural, metabolic and functional signatures. Some of these results, for instance those connected to ultrahigh field CEST-based experiments, are relatively straightforward and in general expectable (Fig. 2). These experiments can be extended to frameworks using relaxation-enhanced and Fourier-encoded manipulations, to transfer magnetization from water and yield magnified signals in 1D and 2D 1H and even 13C NMR experiments of molecular targets (Fig. 3). Another set of experiments explores the use water as potential metabolic marker of development and disease via 2H MRSI (to be shown). Lastly, the discussion will focus on dissolution Dynamic Nuclear Polarization (dDNP) experiments which, instead of polarizing metabolic targets, hyperpolarize water. When suitably performed we find that this can also provide significant NMR enhancements for the signals of biomolecules dissolved in it, particularly of labile groups in metabolites, proteins and nucleic acids (Fig. 4). For better or worse, however, some of these in vitro enhancements are sometimes larger than what could be expected based on existing paradigms.
ABOUT THE SPEAKER
Lucio Frydman undertook postdoctoral studies at the Lawrence Berkeley Laboratory with Alex Pines; then joined the faculty of the University of Illinois at Chicago’s Department of Chemistry in 1992, where he became a Full Professor in 1999. In 2001, he moved to the Weizmann Institute, where he currently heads the Department of Chemical and Biological Physics as well as the Kimmel Institute of Magnetic Resonance and the Clore Institute for High Field Spectroscopy and Imaging. Since 2012 he is also Chief Scientist in Chemistry and Biology and the US National High Magnetic Field Lab. Prof. Frydman’s research focuses on magnetic resonance spectroscopy (NMR) and imaging (MRI).
ABOUT THE 2020-2021 BME WEBINAR SERIES
The Department of Biomedical Engineering at Columbia University is proud to host an annual weekly webinar series on the latest developments and research in Biomedical Engineering. The weekly series takes place on Friday mornings at 11:00AM Eastern and includes a variety of renowned academics from top universities to talk about their specific research and experience.